6IOJ

Glyceraldehyde-3-phosphate dehydrogenase A (apo-form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97917
Spacegroup name	I 41 2 2
Unit cell lengths	120.890, 120.890, 156.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.741 - 2.290
R-factor	0.192
Rwork	0.191
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gad
Data reduction software	xia2
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.7.2)
Refinement software	PHENIX ((1.14_3260:000))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.790	47.790	2.350
High resolution limit [Å]	2.290	10.240	2.290
Rmerge	0.109	0.054	1.602
Rmeas	0.111	0.055	1.633
Rpim	0.022	0.012	0.314
Number of reflections	26233	350	1910
<I/σ(I)>	19.2
Completeness [%]	99.6	98.8	99.9
Redundancy	25.6	21.3	26.5
CC(1/2)	0.999	0.998	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.15M Malic acid, PEG3350 25%