6INZ
Crystal structure of solute-binding protein complexed with unsaturated hyaluronan disaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-28 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.740, 97.501, 132.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.410 - 2.289 |
| R-factor | 0.1622 |
| Rwork | 0.160 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gub |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.645 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.289 | 2.289 |
| Rmerge | 0.076 | 0.510 |
| Number of reflections | 29129 | 1439 |
| <I/σ(I)> | 23.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200mM litium sulfate, 100mM Tris-HCl (pH 8.5), 30% PEG 4000 |
