6INQ
RNA polymerase II elongation complex stalled at SHL(-1) of the nucleosome, with foreign DNA (+1 position)
Replaces: 6A5VEM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | CRYSTALLOGRAPHY |
| Resolution (Å) | 6.9 |
| Software | PHENIX,Coot,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | NONE |
