6INN
Crystal structure of the CysR-CTLD3 fragment of human MR at acidic pH (pH 5.6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-09-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 4 |
| Unit cell lengths | 235.867, 235.867, 129.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.988 - 3.001 |
| R-factor | 0.256 |
| Rwork | 0.256 |
| R-free | 0.27760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xts |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.449 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.184 | 0.473 |
| Number of reflections | 266852 | 13195 |
| <I/σ(I)> | 7.42 | |
| Completeness [%] | 98.4 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.5 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate (pH 5.6), 1.0 M lithium sulfate monohydrate |
