6IN9
Crystal structure of MucB in complex with MucA(peri)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.149, 50.268, 100.137 |
| Unit cell angles | 90.00, 109.05, 90.00 |
Refinement procedure
| Resolution | 44.396 - 1.801 |
| R-factor | 0.1893 |
| Rwork | 0.188 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p4b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.092 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.044 | 0.262 |
| Number of reflections | 61207 | 3024 |
| <I/σ(I)> | 17.8 | 2.1 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Potassium citrate tribasic monohydrate, 20% w/v polyethylene glycol 3350 |
