6IN7
Crystal structure of AlgU in complex with MucA(cyto)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.977853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.784, 72.256, 81.721 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.505 - 1.960 |
R-factor | 0.1789 |
Rwork | 0.178 |
R-free | 0.20110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1or7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.779 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Rpim | 0.090 | 0.276 |
Number of reflections | 23934 | 1053 |
<I/σ(I)> | 27.9 | 9.2 |
Completeness [%] | 99.5 | 90.6 |
Redundancy | 6.2 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2.0 M ammonium sulfate,0.1 M cacodylate pH 6.5, 0.2M sodium chloride |