6IN5

Crystal structure of H5N2 hemagglutinin G228S Q226L mutant with 3SLN from A/chicken/Taiwan/0502/2012

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	110.323, 110.323, 314.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.364 - 2.916
R-factor	0.2225
Rwork	0.219
R-free	0.29110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ykc
RMSD bond length	0.009
RMSD bond angle	1.128
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.364	3.090
High resolution limit [Å]	2.916	2.910
Rmerge	0.084	0.718
Rmeas	0.094	0.804
Rpim	0.041	0.355
Number of reflections	16461
<I/σ(I)>	8.9
Completeness [%]	98.3	97.5
Redundancy	5.1	4.9
CC(1/2)	0.970	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Tris, 0.2M MgCl, 14%PEG8000