6IMW
The complex structure of endo-beta-1,2-glucanase mutant (E262Q) from Talaromyces funiculosus with beta-1,2-glucan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.169, 117.924, 93.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.640 - 2.100 |
R-factor | 0.21098 |
Rwork | 0.210 |
R-free | 0.23567 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6imu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.112 |
Data reduction software | XDS (June 1, 2017) |
Data scaling software | Aimless (0.7.3) |
Phasing software | MOLREP (7.0.014) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.640 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.184 | 0.812 |
Number of reflections | 60060 | 4375 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M citrate buffer (pH 4.0), 0.1M NaCl, 25%(w/v) PEG2000 |