6IMV
The complex structure of endo-beta-1,2-glucanase from Talaromyces funiculosus with sophorose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-23 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.088, 118.104, 93.428 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.710 - 2.000 |
R-factor | 0.18297 |
Rwork | 0.182 |
R-free | 0.20897 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6imu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.105 |
Data reduction software | XDS (June 1, 2017) |
Data scaling software | Aimless (0.7.3) |
Phasing software | MOLREP (7.0.060) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.710 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.128 | 0.452 |
Number of reflections | 69519 | 4430 |
<I/σ(I)> | 9.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M phosphate-citrate buffer (pH 4.6), 0.1M NaCl, 20%(w/v) PEG3350 |