6IMU
The apo-structure of endo-beta-1,2-glucanase from Talaromyces funiculosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-08 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.657, 117.971, 93.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.580 - 2.000 |
| R-factor | 0.22136 |
| Rwork | 0.220 |
| R-free | 0.24807 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.037 |
| Data reduction software | XDS (June 1, 2017) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | CRANK2 (2.0.132) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.580 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.174 |
| Number of reflections | 68894 | 4566 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M citrate buffer (pH 4.1), 0.1M NaCl, 20%(w/v) PEG 2000 |
