6IML
The crystal structure of AsfvLIG:CT1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.244, 53.735, 76.232 |
| Unit cell angles | 90.00, 100.47, 90.00 |
Refinement procedure
| Resolution | 29.884 - 2.350 |
| R-factor | 0.2217 |
| Rwork | 0.219 |
| R-free | 0.26780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ymj |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.490 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.133 | 0.600 |
| Number of reflections | 23122 | 2021 |
| <I/σ(I)> | 15.9 | 1.77 |
| Completeness [%] | 95.5 | 83.7 |
| Redundancy | 3.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M BIS-TRIS pH 5.5, 15% w/v PEG 10000, 0.1 M Ammonium acetate |
