6IMK
The crystal structure of AsfvLIG:CG complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.257, 55.159, 79.875 |
| Unit cell angles | 90.00, 103.15, 90.00 |
Refinement procedure
| Resolution | 29.674 - 2.502 |
| R-factor | 0.2076 |
| Rwork | 0.205 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6imj |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.498 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.107 | 0.480 |
| Number of reflections | 21031 | 2046 |
| <I/σ(I)> | 17.25 | 2.67 |
| Completeness [%] | 98.5 | 96.9 |
| Redundancy | 5.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M BIS-TRIS pH 5.5, 10% w/v PEG 10000, 0.1 M Ammonium acetate |
