6IMA
Crystal Structure of ALKBH1 without alpha-1 (N37-C369)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.064, 56.062, 105.664 |
| Unit cell angles | 90.00, 117.89, 90.00 |
Refinement procedure
| Resolution | 44.332 - 2.593 |
| R-factor | 0.1951 |
| Rwork | 0.190 |
| R-free | 0.23910 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((1.14rc3_3206: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.593 | 7.050 | 2.600 |
| Rmerge | 0.102 | 0.060 | 0.819 |
| Rmeas | 0.113 | 0.067 | 0.900 |
| Rpim | 0.047 | 0.028 | 0.367 |
| Number of reflections | 62965 | 1680 | 1624 |
| <I/σ(I)> | 5.4 | ||
| Completeness [%] | 98.7 | 97.1 | 99.1 |
| Redundancy | 5.7 | 5.3 | 5.9 |
| CC(1/2) | 0.996 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 16% PEG3350, 0.13 M ammonium citrate dibasic |
