6ILT

Structure of Arabidopsis thaliana Ribokinase complexed with ATP and Magnesium ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 42 21 2
Unit cell lengths	99.445, 99.445, 164.952
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.343 - 2.200
R-factor	0.2263
Rwork	0.224
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ilr
RMSD bond length	0.009
RMSD bond angle	1.250
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	40517
<I/σ(I)>	19	1.2
Completeness [%]	93.3
Redundancy	9.3
CC(1/2)	0.998	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M sodium citrate (pH 5.5), 40% (v/v) PEG 600