6ILS
Structure of Arabidopsis thaliana Ribokinase complexed with Ribose and ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 99.460, 99.460, 165.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.833 - 1.800 |
R-factor | 0.2179 |
Rwork | 0.216 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ilr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.096 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.101 | 4.303 |
Number of reflections | 84606 | |
<I/σ(I)> | 29.6 | |
Completeness [%] | 99.8 | |
Redundancy | 13.8 | |
CC(1/2) | 0.999 | 0.427 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M sodium citrate (pH 5.5) , 40% (v/v) PEG 600 |