6ILR
Structure of Arabidopsis thaliana Ribokinase in unligand form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 99.040, 99.040, 165.458 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.469 - 1.972 |
R-factor | 0.2211 |
Rwork | 0.220 |
R-free | 0.25050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rkd |
RMSD bond length | 0.006 |
RMSD bond angle | 0.923 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.972 | 1.980 |
Rmerge | 0.100 | 1.788 |
Number of reflections | 58558 | |
<I/σ(I)> | 23.8 | |
Completeness [%] | 99.8 | |
Redundancy | 13 | |
CC(1/2) | 0.999 | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M sodium citrate (pH 5.5), 40 % (v/v) PEG 600 |