6ILG

CRYSTAL STRUCTURE OF BAT MHC CLASS I PTAL-N*01:01 FOR 2.6 ANGSTROM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-06-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	72.876, 86.440, 135.121
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.561 - 2.600
R-factor	0.2166
Rwork	0.213
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.644
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.120	2.600
High resolution limit [Å]	1.930	2.540
Rmerge	0.285	0.628
Number of reflections	14539	1065
<I/σ(I)>	19
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Lithium sulfate monohydrate, 0.1M BIS-TRIS pH 6.5, 25%(w/v) Polyethylene glycol 3,350