6IL7
Structure of Enterococcus faecalis (V583) alkylhydroperoxide reductase subunit F (AhpF) C503A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 134.101, 134.101, 134.101 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.21723 |
Rwork | 0.215 |
R-free | 0.26719 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h29 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.088 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.069 | 0.252 |
Number of reflections | 14035 | 1425 |
<I/σ(I)> | 22.4 | 7.3 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 11.2 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.1 M Bis-Tris (pH 6.5), 1.5 M ammonium sulfate and 0.1 M sodium chloride |