6IKX
UDP-glucose pyrophosphorylase from acinetobacter baumanii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.598, 118.598, 108.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.720 - 2.200 |
| R-factor | 0.2181 |
| Rwork | 0.214 |
| R-free | 0.29240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j49 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.107 | 0.072 | 0.478 |
| Rmeas | 0.112 | 0.074 | 0.526 |
| Rpim | 0.029 | 0.019 | 0.209 |
| Total number of observations | 446063 | ||
| Number of reflections | 39722 | 2173 | 1932 |
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.4 | 98.5 | 98.4 |
| Redundancy | 11.2 | 15.7 | 5.5 |
| CC(1/2) | 0.998 | 0.078 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 287 | 1.5M Ammonium citrate, 0.1M BIS-TRIS pH 6.13 and 0.1M NaCl |
