6IK8
Crystal structure of tomato beta-galactosidase (TBG) 4 in complex with beta-1,6-galactobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.940, 95.950, 158.959 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.020 - 2.800 |
R-factor | 0.18501 |
Rwork | 0.182 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w5g |
RMSD bond length | 0.006 |
RMSD bond angle | 1.376 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.152 | 0.755 |
Number of reflections | 35773 | 1744 |
<I/σ(I)> | 12.413 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 16% (w/v) PEG10000, 0.1M HEPES |