6IK5

Crystal structure of tomato beta-galactosidase (TBG) 4 in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000000
Spacegroup name	P 21 21 21
Unit cell lengths	92.136, 95.335, 158.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.280 - 1.820
R-factor	0.17344
Rwork	0.172
R-free	0.20867
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w5g
RMSD bond length	0.015
RMSD bond angle	1.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.280	1.885
High resolution limit [Å]	1.820	1.820
Rmerge	0.052	0.615
Rmeas	0.083	0.782
Rpim	0.031	0.297
Number of reflections	123244	11826
<I/σ(I)>	23.4	2.68
Completeness [%]	98.3	95.47
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	16% (w/v) PEG10000, 0.1M HEPES