6IK5
Crystal structure of tomato beta-galactosidase (TBG) 4 in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.136, 95.335, 158.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.280 - 1.820 |
| R-factor | 0.17344 |
| Rwork | 0.172 |
| R-free | 0.20867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5g |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.280 | 1.885 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.052 | 0.615 |
| Rmeas | 0.083 | 0.782 |
| Rpim | 0.031 | 0.297 |
| Number of reflections | 123244 | 11826 |
| <I/σ(I)> | 23.4 | 2.68 |
| Completeness [%] | 98.3 | 95.47 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 16% (w/v) PEG10000, 0.1M HEPES |
