6IJU

The DNA binding domain of a response regulator ArlR from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 64 2 2
Unit cell lengths	67.233, 67.233, 153.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.000 - 2.400
R-factor	0.2129
Rwork	0.211
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ys6
RMSD bond length	0.007
RMSD bond angle	0.878
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.566
Number of reflections	7638	406
<I/σ(I)>	50.1
Completeness [%]	99.9
Redundancy	36.6
CC(1/2)	0.875	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1M sodium acetate trihydrate pH4.5 , 2.0M ammonium sulfate