6IJ2
Crystal structure of a standalone versatile EAL protein from Vibrio cholerae O395 - 5'-pGpG bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-23 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.483, 42.711, 158.470 |
| Unit cell angles | 90.00, 94.94, 90.00 |
Refinement procedure
| Resolution | 51.511 - 1.700 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gfx |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.797 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.630 | 52.630 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.310 | 1.700 |
| Rmerge | 0.038 | 0.029 | 0.600 |
| Rmeas | 0.045 | 0.037 | 0.715 |
| Rpim | 0.024 | 0.023 | 0.384 |
| Total number of observations | 361603 | ||
| Number of reflections | 106944 | 709 | 5230 |
| <I/σ(I)> | 13.8 | ||
| Completeness [%] | 98.5 | 96.2 | 99.5 |
| Redundancy | 3.4 | 2.9 | 3.4 |
| CC(1/2) | 0.998 | 0.998 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG 4000, sodium acetate, ammonium acetate |
