6IIM
USP14 catalytic domain with IU1-206
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-11-25 |
| Detector | RIGAKU |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.310, 81.186, 108.511 |
| Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.210 |
| R-factor | 0.2083 |
| Rwork | 0.206 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ayn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.178 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 48730 | 4594 |
| <I/σ(I)> | 19.46 | |
| Completeness [%] | 95.9 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, NH4F, Cscl, glycine |
