6IIL
USP14 catalytic domain bind to IU1-47
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-11-10 |
Detector | RIGAKU |
Wavelength(s) | 0.984 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.427, 81.404, 108.161 |
Unit cell angles | 90.00, 94.62, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.1897 |
Rwork | 0.188 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ayn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.221 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | SHELXL (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 48528 | 3434 |
<I/σ(I)> | 33.05 | |
Completeness [%] | 93.7 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, NH4F, glycine, Cscl |