6IIG

crystal structure of H5N2 hemagglutinin G228S mutant With 6SLN from A/chicken/Taiwan/0502/2012

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	111.325, 111.325, 313.945
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.384 - 3.208
R-factor	0.2257
Rwork	0.222
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ykc
RMSD bond length	0.010
RMSD bond angle	1.222
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.118	0.596
Rmeas	0.133	0.682
Rpim	0.062	0.325
Number of reflections	12660
<I/σ(I)>	5.5
Completeness [%]	100.0
Redundancy	4.6	4.3
CC(1/2)	0.967	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Tris, 0.2M MgCl, 14%PEG8000