6IIG
crystal structure of H5N2 hemagglutinin G228S mutant With 6SLN from A/chicken/Taiwan/0502/2012
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 111.325, 111.325, 313.945 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.384 - 3.208 |
| R-factor | 0.2257 |
| Rwork | 0.222 |
| R-free | 0.28990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ykc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.222 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.118 | 0.596 |
| Rmeas | 0.133 | 0.682 |
| Rpim | 0.062 | 0.325 |
| Number of reflections | 12660 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.6 | 4.3 |
| CC(1/2) | 0.967 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Tris, 0.2M MgCl, 14%PEG8000 |
