6II4
Crystal structure of H7 hemagglutinin from A/Anhui/1/2013 in complex with a human neutralizing antibody L4A-14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | H 3 |
| Unit cell lengths | 127.484, 127.484, 429.649 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.044 - 3.300 |
| R-factor | 0.2292 |
| Rwork | 0.227 |
| R-free | 0.27620 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1-2575_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.055 | |
| Number of reflections | 39511 | |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 291.15 | 0.2M Ammonium citrate tribasic pH7.0, 20%, 30 w/v PEG 3350 |
