6II4
Crystal structure of H7 hemagglutinin from A/Anhui/1/2013 in complex with a human neutralizing antibody L4A-14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97930 |
Spacegroup name | H 3 |
Unit cell lengths | 127.484, 127.484, 429.649 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.044 - 3.300 |
R-factor | 0.2292 |
Rwork | 0.227 |
R-free | 0.27620 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.607 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1-2575_1692: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rpim | 0.055 | |
Number of reflections | 39511 | |
<I/σ(I)> | 14.8 | |
Completeness [%] | 99.9 | |
Redundancy | 6 | |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 291.15 | 0.2M Ammonium citrate tribasic pH7.0, 20%, 30 w/v PEG 3350 |