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6II0

Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron sitePAL/PLS
Beamline7A (6B, 6C1)
Temperature [K]100
Detector technologyCCD
Collection date2018-02-26
DetectorADSC QUANTUM 270
Wavelength(s)0.9793
Spacegroup nameP 61
Unit cell lengths78.640, 78.640, 450.008
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution50.000 - 2.360
R-factor0.18517
Rwork0.182
R-free0.23662
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6ii6
RMSD bond length0.011
RMSD bond angle1.436
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwareREFMAC (5.8.0158)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.400
High resolution limit [Å]2.3602.360
Rmeas0.643
Rpim0.273
Number of reflections608063219
<I/σ(I)>6.1
Completeness [%]97.3
Redundancy5
CC(1/2)0.770
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP27712% PEG 3000, 0.1M Na2HPO4/citric acid (pH 4.2), 0.4M NaCl, 10% glycerol

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