6II0
Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 |
Unit cell lengths | 78.640, 78.640, 450.008 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.360 |
R-factor | 0.18517 |
Rwork | 0.182 |
R-free | 0.23662 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ii6 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.436 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmeas | 0.643 | |
Rpim | 0.273 | |
Number of reflections | 60806 | 3219 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 97.3 | |
Redundancy | 5 | |
CC(1/2) | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 12% PEG 3000, 0.1M Na2HPO4/citric acid (pH 4.2), 0.4M NaCl, 10% glycerol |