6IHJ
Crystal structure of Drosophila Nxf1 NTF2 domain in complex with Nxt1/p15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97854 |
| Spacegroup name | P 62 |
| Unit cell lengths | 152.204, 152.204, 49.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.764 - 2.700 |
| R-factor | 0.2284 |
| Rwork | 0.224 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jkg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.714 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.125 | 0.430 |
| Number of reflections | 18389 | 1822 |
| <I/σ(I)> | 22.1 | 2.33 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 13.5 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 16%(w/v) PEG 8000, 40mM Potassium phosphate dibasic and 20%(v/v) Glycerol |
