6IHH
Crystal structure of RasADH F12 from Ralstonia.sp in complex with NADPH and A6O
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.687, 118.687, 73.009 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.350 - 1.800 |
| R-factor | 0.16828 |
| Rwork | 0.166 |
| R-free | 0.20393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bms |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.576 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.870 | 1.800 |
| Rmerge | 0.099 | 0.056 | 0.613 |
| Rmeas | 0.105 | 0.059 | 0.650 |
| Rpim | 0.033 | 0.019 | 0.214 |
| Total number of observations | 565706 | ||
| Number of reflections | 55225 | 5776 | 5156 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 99.4 | 100 | 93.6 |
| Redundancy | 10.2 | 10.2 | 8.6 |
| CC(1/2) | 0.998 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Ammonium Sulfate, Bis-Tris, PEG3350 |
