6IHG

N terminal domain of Mycobacterium avium complex Lon protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 31 2 1
Unit cell lengths	58.631, 58.631, 118.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.823 - 2.397
R-factor	0.2296
Rwork	0.227
R-free	0.26820
Structure solution method	SAD
Starting model (for MR)	6IHF
RMSD bond length	0.009
RMSD bond angle	1.091
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.15rc2_3428: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.397	2.400
Rmerge	0.076	0.630
Number of reflections	8942	457
<I/σ(I)>	3
Completeness [%]	91.8
Redundancy	18.3
CC(1/2)		0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		295	Sodium formate,Sodium acetate trihydrate