6IGX
Crystal structure of human CAP-G in complex with CAP-H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 122.433, 61.998, 130.885 |
Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
Resolution | 19.810 - 2.995 |
R-factor | 0.215 |
Rwork | 0.212 |
R-free | 0.27170 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.684 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.160 |
High resolution limit [Å] | 2.995 | 2.995 |
Rmerge | 0.069 | 0.552 |
Rmeas | 0.082 | 0.653 |
Number of reflections | 39494 | 6128 |
<I/σ(I)> | 13.8 | 2.07 |
Completeness [%] | 98.6 | 98 |
Redundancy | 3.35 | 3.45 |
CC(1/2) | 0.998 | 0.721 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, HEPES-NaOH pH 7.5, Ethylen glycol, Magnesium chloride |