6IGW
MPZL1 mutant - S86G, V145G, Q146K,P147T,G148S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 39.991, 39.991, 193.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.955 - 1.979 |
R-factor | 0.2413 |
Rwork | 0.241 |
R-free | 0.24720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oai |
RMSD bond length | 0.011 |
RMSD bond angle | 1.209 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.979 | 1.979 |
Rpim | 0.028 | 0.209 |
Number of reflections | 10881 | 561 |
<I/σ(I)> | 20.28 | |
Completeness [%] | 99.8 | |
Redundancy | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 20mM Tris-HCl pH 8.5, 1M Li2SO4, 1.8%(w/v) PEG8000 |