6IGT
MPZL1 mutant - V145G, Q146K, P147T and G148S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.425, 40.417, 195.223 |
Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
Resolution | 40.425 - 2.404 |
R-factor | 0.2219 |
Rwork | 0.220 |
R-free | 0.25150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oai |
RMSD bond length | 0.009 |
RMSD bond angle | 1.143 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.046 | 0.349 |
Number of reflections | 23051 | 1330 |
<I/σ(I)> | 14.89 | |
Completeness [%] | 98.6 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 50mM MES pH5.6, 100 mM (NH4)2SO4, 10mM MgCl2, 20% (w/v) PEG8000 |