6IGO

Crystal structure of myelin protein zero-like protein 1 (MPZL1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 1 21 1
Unit cell lengths	53.462, 121.703, 70.395
Unit cell angles	90.00, 100.24, 90.00

Refinement procedure

Resolution	48.291 - 2.746
R-factor	0.2267
Rwork	0.225
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oai
RMSD bond length	0.010
RMSD bond angle	1.094
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2400: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.746	2.746
Rpim	0.056	0.373
Number of reflections	22192	1700
<I/σ(I)>	11.87
Completeness [%]	99.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100mM Tris-HCl pH 8.5, 1.15M (NH4)2HPO4