6IGN

The crystal structure of Kif5b stalk 1 coiled-coil region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 42 2 2
Unit cell lengths	50.559, 50.559, 162.603
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.971 - 3.451
R-factor	0.2787
Rwork	0.274
R-free	0.31750
Structure solution method	SAD
RMSD bond length	0.001
RMSD bond angle	0.425
Data reduction software	HKL-2000 (v)
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	PHENIX ((1.14_3228: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.510
High resolution limit [Å]	3.450	3.450
Rmerge	0.063	0.583
Number of reflections	3101
<I/σ(I)>	46.389
Completeness [%]	86.9
Redundancy	19.2	17.2
CC(1/2)	0.961	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	298	0.05 M magnesium chloride,5% PEG 8000, 40% MPD, 0.1M sodium cacodylate pH6.2