6IGD

Crystal structure of HPV58/33 chimeric L1 pentamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2016-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	153.686, 105.833, 154.713
Unit cell angles	90.00, 99.55, 90.00

Refinement procedure

Resolution	40.800 - 2.500
R-factor	0.1837
Rwork	0.182
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y9e
RMSD bond length	0.004
RMSD bond angle	0.669
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	169844	8408
<I/σ(I)>	11.1
Completeness [%]	99.8
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2M potassium thiocyanate, 23% (w/v) PEG 3350