6IGA
Crystal structure of argininosuccinate lyase from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97778 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 129.155, 129.155, 200.137 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.673 - 2.776 |
R-factor | 0.2208 |
Rwork | 0.219 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hy1 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.942 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.776 | 2.800 |
Rmerge | 0.142 | 0.443 |
Number of reflections | 43973 | 2273 |
<I/σ(I)> | 7.66 | |
Completeness [%] | 95.8 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium tartrate dibasic, pH 7.0, 20% (w/v) polyethylene glycol 3350 |