6IG6

Crystal structure of lysozyme delivered in polyacrylamide using x-ray free electron laser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	PAL-XFEL BEAMLINE NCI
Synchrotron site	PAL-XFEL
Beamline	NCI
Temperature [K]	296
Detector technology	CCD
Collection date	2018-06-15
Detector	RAYONIX MX225-HS
Wavelength(s)	1.2782
Spacegroup name	P 43 21 2
Unit cell lengths	79.600, 79.600, 38.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.043 - 1.700
R-factor	0.1976
Rwork	0.196
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4et8
RMSD bond length	0.008
RMSD bond angle	0.941
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	14007	1353
<I/σ(I)>	4.32	1.39
Completeness [%]	100.0	100
Redundancy	965.3	676.7
CC(1/2)	0.990	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, PEG 8000, NaCl