6IG4
Structure of mitochondrial CDP-DAG synthase Tam41, delta 74
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 173.15 |
Detector technology | PIXEL |
Collection date | 2015-09-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 109.069, 116.538, 108.548 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.677 - 2.261 |
R-factor | 0.1833 |
Rwork | 0.181 |
R-free | 0.22010 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.126 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.124 | 0.468 |
Number of reflections | 32658 | 1605 |
<I/σ(I)> | 23.3 | 5.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13 | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289.15 | 15% Tacsimate, pH 7.0, 20 mM CdCl2, 10 mM K2Pt(CN)4 |