6IFX
Crystal structure of chimeric KsgA with loop 12 from Erm
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.693, 79.499, 130.115 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.840 - 3.800 |
R-factor | 0.30761 |
Rwork | 0.304 |
R-free | 0.38502 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.571 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.840 | 3.897 |
High resolution limit [Å] | 3.800 | 3.800 |
Number of reflections | 7342 | |
<I/σ(I)> | 13.3 | 5.57 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Sodium chloride, 0.05M Sodium cacodylate trihydrate pH 6.0, 10% w/v Polyethylene glycol 4000, 0.0005M Spermine |