6IFT
KsgA from Bacillus subtilis in complex with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.200, 81.970, 47.120 |
| Unit cell angles | 90.00, 112.02, 90.00 |
Refinement procedure
| Resolution | 19.280 - 1.900 |
| R-factor | 0.17913 |
| Rwork | 0.177 |
| R-free | 0.23495 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.043 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.280 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 25102 | |
| <I/σ(I)> | 12 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% tacsimate pH 6.0, 20% PEG 3350 |
