6IFS
KsgA from Bacillus subtilis 168
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.850, 64.180, 101.390 |
Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
Resolution | 19.640 - 2.270 |
R-factor | 0.17926 |
Rwork | 0.177 |
R-free | 0.23757 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.770 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.640 | 2.323 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 26353 | |
<I/σ(I)> | 16.3 | 4.9 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 7.5 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Succinic acid, pH 7.0, 12% PEG 3350 |