6IFS
KsgA from Bacillus subtilis 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.850, 64.180, 101.390 |
| Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
| Resolution | 19.640 - 2.270 |
| R-factor | 0.17926 |
| Rwork | 0.177 |
| R-free | 0.23757 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.770 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.640 | 2.323 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 26353 | |
| <I/σ(I)> | 16.3 | 4.9 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Succinic acid, pH 7.0, 12% PEG 3350 |
