6IFN
Crystal structure of Type III-A CRISPR Csm complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97775 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 123.543, 82.334, 161.358 |
Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
Resolution | 48.680 - 2.900 |
R-factor | 0.20337 |
Rwork | 0.201 |
R-free | 0.24428 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ifl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.388 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.069 | 0.446 |
Number of reflections | 70612 | 6836 |
<I/σ(I)> | 8 | |
Completeness [%] | 97.2 | 94.7 |
Redundancy | 4.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M sodium citrate tribasic dihydrate, pH 5.0, 20% w/v polyethylene glycol 20,000. |