6IFB
Structure of rhamnose bound beta-trefoil lectin from Entamoeba histolytica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.194, 62.377, 67.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.780 - 1.370 |
R-factor | 0.159 |
Rwork | 0.158 |
R-free | 0.17660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.793 |
Data reduction software | XDS (2018-04-27) |
Data scaling software | XDS (2018-04-27) |
Phasing software | PHASER (2.7.0) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.780 | 1.419 |
High resolution limit [Å] | 1.370 | 1.370 |
Number of reflections | 50944 | 7723 |
<I/σ(I)> | 14.58 | 2.3 |
Completeness [%] | 99.0 | 94.3 |
Redundancy | 8.47 | 7.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 20% iso-propanol, 0.1M MES monohydrate, pH 6.0, 20% PEG monomethyl ether 2000 |