6IF7

Crystal Structure of AA10 Lytic Polysaccharide Monooxygenase from Tectaria macrodonta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	79.221, 79.221, 68.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.020 - 2.200
R-factor	0.191
Rwork	0.189
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oy7
RMSD bond length	0.011
RMSD bond angle	1.466
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.020	50.020	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.065	0.042	0.261
Number of reflections	11475		1928
<I/σ(I)>	16.1
Completeness [%]	99.0	99.7	90.6
Redundancy	4.9	5.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1M MES PH 6.5, 1.6M Magnesium sulphate