6IF6
Structure of the periplasmic domain of SflA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2014-11-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 66.900, 66.900, 59.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.898 - 1.900 |
R-factor | 0.1804 |
Rwork | 0.176 |
R-free | 0.22070 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.078 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.900 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.078 | 0.583 |
Number of reflections | 12475 | 833 |
<I/σ(I)> | 15.6 | 3.2 |
Completeness [%] | 99.8 | 98.3 |
Redundancy | 8.6 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-NaOH pH 7.5, 0.8M sodium potassium phosphate |