6IER
Apo structure of a beta-glucosidase 1317
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-20 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 62 |
| Unit cell lengths | 122.990, 122.990, 48.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.747 - 2.246 |
| R-factor | 0.2076 |
| Rwork | 0.206 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gnx |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.701 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 2.370 |
| High resolution limit [Å] | 2.240 | 2.250 |
| Rmerge | 0.100 | 0.293 |
| Number of reflections | 20173 | 2775 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 99.1 | 94.3 |
| Redundancy | 4.8 | |
| CC(1/2) | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris-HCl (pH 8.5), 25% PEG 3350 |
