6IEK
Structure of RVFV Gn and human monoclonal antibody R12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.756, 108.070, 107.418 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 48.271 - 2.700 |
| R-factor | 0.2267 |
| Rwork | 0.225 |
| R-free | 0.25800 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 61396 | 6102 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M TRIS, PH8, 1.2M Na/K tartrate |
