6IEB

Structure of RVFV Gn and human monoclonal antibody R15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	69.002, 70.521, 99.330
Unit cell angles	85.09, 84.71, 70.70

Refinement procedure

Resolution	38.560 - 2.409
R-factor	0.1974
Rwork	0.195
R-free	0.24580
RMSD bond length	0.011
RMSD bond angle	1.172
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.051	0.540
Number of reflections	64619
<I/σ(I)>	18.6	3.1
Completeness [%]	95.8	97.3
Redundancy	3.2	3.2
CC(1/2)	0.991	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	PH6.5, 0.1M Amino acids, 0.1M Buffer System 1, 30.00% v/v P500MME/P20K